Welcome to UMARS:EEJ Server

UMARS:EEJ The purpose of UMARS:EEJ is to identify novel alternative spliced exon-exon junctions from un-mappable reads. The sequences of all possible exon-exon junctions (EEJs) of 21 species were collected in advance. In UMARS:EEJ, uploaded reads are subject to mapping to EEJs. To avoid random sequence match, besides our mapping criteria (see Materials and Methods), a mapping match must overlap both exons for at least five nucleotides, not skewing too much to any exon. Following the mapping procedure, UMARS tabulated detected EEJs and expression levels of them. The detected EEJs were reported as either continuous EEJs or discrete ones. Continuous EEJs represent known mRNA transcripts. However, discrete EEJs could represent novel splicing isoforms.